Abstract
The article presents molecular genetic description of isolates of Mycobacterium tuberculosis of 178 new patients and 78 patients who had been previously treated obtained in 2009-2011 on the territory of Urals. PCR in real time and MIRU-VNTR typing of 15 loci detected prevalence of M. tuberculosis of Beijing genotype in the both groups of patients, however the part of Beijing isolates was significantly higher in the group of patients who had been previously treated compared to new patients: 80.8% versus 55.1% (p = 0.0002). IS6110-RFLP-typing showed that the majority of Beijing isolates belonged to B0 clone, clinically and epidemiologically significant in Russia. It was found out that M. tuberculosis Beijing carrying mutations rpoB Ser531→Leu and katG Ser315→Thr played the major role in MDR tuberculosis transmission in Urals. Non-Beijing group was represented by isolates of various spoligotypes and MIRU-VNTR types among which the representatives of the following globally common genetic groups prevailed: LAM (LAM9, T5_RUS), URAL (Н3), Haarlem (H1, X), Т (Т1).
Highlights
Представлена молекулярно-генетическая характеристика изолятов Mycobacterium tuberculosis, полученных в 2009-2011 гг. на территории Урала от 178 ранее не леченных и 78 ранее леченных больных туберкулезом
PC R in real tim e and M IRU -VNTR typing of 15 loci detected prevalence of M. tuberculosis of Beijing genotype in the both groups of patients, the part of Beijing isolates was significantly higher in the group of patients who had been previously treated compared to new patients: 80.8% versus 55.1% (p = 0.0002)
IS6110-RFLP-typing showed th at the majority of Beijing isolates belonged to B0 clone, clinically and epidemiologically significant in Russia
Summary
Представлена молекулярно-генетическая характеристика изолятов Mycobacterium tuberculosis, полученных в 2009-2011 гг. на территории Урала от 178 ранее не леченных и 78 ранее леченных больных туберкулезом. П Ц Р в режиме реального времени и M IRU -VNTR-типирование по 15 локусам вы явили доминирование M. tuberculosis генотипа Beijing в обеих группах пациентов, однако доля изолятов Beijing у ранее леченных больных существенно превыш ала таковую в группе ранее не леченных больных: 80,8% против 55,1% (p = 0,0002). Установлено, что M. tuberculosis Beijing, несущие мутации rpoB S er531^L eu и katG S er315^T hr, играют ключевую роль в распространении туберкулеза с М ЛУ возбудителя на Урале.
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