Abstract
Коефіцієнти проникності мембран ентероцитів миші для води і кріопротекторів
Highlights
Osmotic response of cells and transport properties of membrane are essential for cryobiological research in terms of choosing the optimal conditions to cryopreserve the specific cell type
Changes in electrolyte concentration in murine enterocytes and the dynamics of murine enterocyte saturation with cryoprotectant during their contact with 1 M solutions are shown in Figs. 2, 3
This parameter depends on the ratio of filtration and permeability coefficients in the medium of corresponding cryoprotectant
Summary
The experiments in animals were performed according to the General Ethical Principles of Experiments in Animals, approved by the 5th National Congress of Bioethics (Kyiv, 2013) and agreed with the statements of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986). FVwτas0hc=ee–r eaiVSnyr0ei=tkaisVa ol/−fV1v0c–a–elvulraleellmuaoeteifsvmwecbecitrlehlallntvivemoo;lleuLumdmp iem–e;;eVcτnews–li=loc emnVlSs0le;vLmSpob–RlυursTsmau n−er1e-;, filtration coefficient; R – universal gas constant; T – aobf sdoilsustoelvteemd psuebrasttuarnec;eυpse(nυekt)ra–tipnagrt(inalonm-opleanrevtroaltuinmge) fthicrioeungthofthtehececlellml memembrbarnaen;ekrse–latpievremteoabpielniteytrcaotienfgdissolved substance; σs – reflex coefficient of cell membrane, relative to penetrating dissolved substance; ∆πs і ∆πk – are the transmembrane differentials of normalised osmotic pressure of substances penetrating and non-penetrating through cell membrane, respectively; the subscripts s, k and w denote the values, referred to dissolved substances (penetrating and non-peneпроблемы криобиологии и криомедицины problems of cryobiology and cryomedicine том/volume 26, No/issue 3, 2016 мембрани; Lp – коефіцієнт фільтрації клітинної мембрани; R – універсальна газова константа; T – мабослоярлнюитйноаб’тєеммппреорнаиткуарюач;оυї (sн(еυпk)ро–нипкаарюцчіоаїл)ькнриізйь мфеіцмібєрнатнпирколніиткиннорсотізчкилніетнионїнорїечмоевмибнриа; нkиs. Результати та обговорення Для розрахунку коефіцієнта фільтрації Lp та коефіцієнтів проникності клітинних мембран для кріопротекторів експериментально визначені часові залежності об’єму клітин за їх контакту з гіпертонічними розчинами кріопротекторів апроксимували рішеннями системи рівнянь (1)–(3) Nk0 – volume fraction membrane substances inside a cell or osmotically inactive cell volume We determined this value previously [11], for murine enterocytes it made 0.51 ± 0.06
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