초고압 추출 처리에 의한 더덕 및 발효더덕의 항산화 증진

Abstract

This study was designed to evaluate antioxidant activity of low-quality Codonopsis lanceolata treated by fermentation process followed by hydraulic high pressure extraction. C. lanceolata was subjected to 5,000 bar for 30 min at 25℃. The highest phenolics content was observed in the combined treatment of fermentation and high pressure extraction (35.11 ㎎/100 g), followed by high pressure extraction alone (14.78 ㎎/100 g) and conventional extraction (14.56 ㎎/100 g). The content of flavonoids followed the similar pattern as that of total phenolics, showing 280.86 ㎎/100 g of C. lanceolata treated by the combined fermentation with high pressure extraction whereas 193.05 ㎎/100 g of C. lanceolata treated by the conventional extraction. The DPPH scavenging activity was 69.32% at 0.6 ㎎/mL of C. lanceolata treated by the combined process, while the DPPH scavenging activities of C. lanceolata treated by high pressure extraction alone and the conventional extraction were 60.35% and 30.92%, respectively. The highest reducing power of C. lanceolata extract (1.0 ㎎/mL) was observed at the combined treatment (0.926), followed by high pressure extraction alone (0.881) and the conventional extraction (0.733). The combination of fermentation with high pressure extraction significantly increased the contents of phenolics and flavonoids and also enhanced the antioxidant activity. Therefore, the combined application of fermentation and high pressure extraction can be an alternative technique for the extraction of C. lanceolata. These results provide useful information for enhancing biological properties of low-quality C. lanceolata.