흰쥐에서 고용량 아질산이온 함유 마늘 발효농축액에 의한 뇌혈류 변화

Abstract

Nitrate–nitrite–nitric oxide (NO) pathway is a major alternative source of NO and is essential for NO - dependent physiological functions in body. Food supplements having nitrate/nitrite can improve metabolic syndromes including hypertension through antioxidant activity or vasodilation. The purpose of this study was to observe the effects of fermented garlic (F. garlic) having high concentration of NO₂ SUP-/SUP on changes in blood flow and nitric oxide synthesis in the cerebral cortex of rodents. The generation of nitric oxide detected by a chemi-luminescence detector was higher in F. Garlic compared with NaNO₂ solution under artificial gastric juice with pH 2.0. Ether F. garlic or NaNO₂ diluted with artificial cerebrospinal fluid was directly applied into around the needle probe of laser Doppler flow meter that was located on epidural surface of the cortex. Direct application of F. garlic resulted in increase of cerebral blood flow detected by a laser Doppler flow meter with a dose-dependent manner. Compared with NaNO₂ solution, F. garlic produced changes in cerebral blood flow at lower concentration of NO₂SUP-/SUP. Pretreatment of methylene blue, a guanylyl cyclase inhibitor prevented upregulation of cerebral blood flow by the treatment of F. garlic. In addition, the application of F. garlic with 250, 500ppm of NO₂SUP-/SUP caused significantly the production of NO in the cortical tissue but NaNO₂ solution with 500ppm of NO₂SUP-/SUP did not. In summary, these results suggested that F. garlic with high content of NO₂SUP-/SUP induce increase in cerebral blood flow through nitric oxide-dependent signal pathway.