Экспериментальная полная транссекция спинного мозга и его биоинженерная реконструкция

Abstract

The work represents the original research devoted to cellular and issue reconstruction of a spinal cord injury in adult rats with an experimental vertebral-spinal trauma. After performing a laminectomy, partial and full mechanical intersection of the spinal cord at the IX thoracic vertebrae in the spinal implanted medical devices containing bovine collagen and crab chitosan, sulfated and non-sulfated glycosaminoglycans, full nutrient medium, conditioned nutrient medium with neurotrophic factors in the growth of brain cells mouse embryos and embryonic mouse stem cells, N2 neuronal additive, retinoic acid. The dynamics neurologic status in animals has shown essential reduction of deficiency on BBB scale during 20 weeks of the postoperative period. The indirect immune-fluorescence method of a spinal cord cells and histological sections confirms presence of active introduction of cells of a parent spinal cord into implant, high viability of the replaced cells of the mouse during all period of supervision, formation in 1 week after operation of progenitor neuronal cells with expression of neurotransmitters. This change is accompanied by a partial recovery of motor, sensory and vegetative functions of the spinal cord, reduction in the level of neuro-deficit of 5, 6 points on a scale of BBB. Transplantation of collagen-chitosan matrix containing 100 thousand cells, neuronal precursors leads for 20 weeks of observation to preserve their viability, formation of numerous neurons, creating intra-synaptic correlation associated to early expression of neurotransmitters, significant restoration of motor and sensory functions of the spinal cord, reaching a level of reduction neuro-deficit equal to 19.5 points on a scale of BBB.