К ВОПРОСУ О МЕТОДИКЕ КУЛЬТИВИРОВАНИЯ МОРСКИХ И СОЛОНОВАТОВОДНЫХ ИНФУЗОРИЙ

Abstract

The paper shows the results of the study of cultivating marine and brackishwater ciliates conducted at the experimental centre “Zavetnoe” (the Kerch Strait) in 2008-2019. The main parameters for the ciliates cultivation have been defined and the effectiveness of nutrient mixtures for their feeding have been analyzed. Planktonic ciliates Euplotes affinis Dujardin, 1842; Euplotes charon O. F. Müller, 1786; Mesodinium pulex Claparede et Lachmann, 1858 were taken as the research material. Growing was carried out under the tent and in the open air, in ambient conditions, in cultivation containers in broad daylight and with artificial lighting at night. Coral-shell fractions in 5-7 kg/m2 were put on the bottom of the pools as the underlying layer. The density of seeding the uterine culture in E. affinis was 5-10 ind./ml, in E. charon and M. pulex - 1-2 ind./ml. Cultivation of ciliates was carried out in spring and autumn. According to the results of the study, the feed is better used in the form of water-soluble nutrient mixtures. Due to the fact that the nutrient ingredients are in a dissolved state, they are absorbed faster by organisms. This leads to the continuous reproduction of the culture of populations, which helps to grow ciliates even at suboptimal temperatures. Nutrient mixtures are recommended to be added to the culture containers 15-20 days before the introduction of the uterine culture. It has been stated that the increased density of ciliates occurs regardless of the composition of the feed; the density of ciliates always depends on the composition of the nutrient mixture. The best results are obtained when using a nutritious mixture with horse manure and dry banana skin.