Abstract
ABSTRACT Angiotensin-Iconverting enzyme(ACE)inhibitor was isolated from beef by-products. The beef by- product hydrolysates prepared with various proteases were tested for the inhibitory effects against ACE. The proteases used were proteinase A from bakers yeast, protease type OIfungal and thermolysin. The maximum inhibitory effect was observed after hydrolysis for 12hrs(beef heart) and 24hrs(beef spleen), respectively. After gel filtration, IC 50 value was 0.37mg/Lin beef heart and 1.84mg/Lin beef spleen. After RP-HPLC, the IC 50 value of peak 1, peak 2, peak 3 and peak-4 were 0.28mg/L, 0.26mg/L, 0.25mg/Land 0.35mg/L, respectively. In the results of amino acid composition of peak 1, peak 2, peak 3 and peak 4, it was observed that peak 1 was consisted mainly of glycine and methionine, peak 2 was proline, cystine and methionine, peak 3 was proline and peak 4 was alanine, methionine and leucine. In conclusion, beef heart hydrolysate treated with thermolysin+ proteinase A was shown to have the highest inhibitory effect for 12hrs incubation at 37.(
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