Abstract
Роль тРНКПро в претрансферном редактировании аланина пролил-тРНК синтетазой
Highlights
Pretransfer and posttransfer editing were experimentally separated by site-directed mutagenesis
Results. tRNA-dependent pretransfer editing is characterized by three-fold higher velocity than tRNA-independent one
The efficiency of the process depends on the presence of 2'-hydroxyl group of A76 tRNAPro
Summary
Aim. To characterize the process of tRNA-dependent pretransfer editing of alanine by prolyl-tRNA synthetase of bacteria Enterococcus faecalis (ProRS Ef). Velocity of the editing processes in vitro was determined by ATP hydrolysis by ProRS Ef. Pretransfer and posttransfer editing were experimentally separated by site-directed mutagenesis. TRNA-dependent pretransfer editing is characterized by three-fold higher velocity than tRNA-independent one.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
