살균제 효과 검정을 위한 고추 탄저병의 실내 열매 검정법

Abstract

Abstract This study was performed to investigate the effect of conidial density, wetness period andtemperature on conidial germination, appressoria formation and disease incidence. While there was notsignificantly correlated between conidial density and temperature, and conidial germination and appressoriaformation, there was a significant correlation between those factors and disease incidence. The longer wetnessperiod was, the higher the ratio of conidial germination, appressoria formation and the disease incidence was.The optimum conidial density, temperature and wetness period was 1× 10 6 conidia mL Š 1 , 30 o C and 5 days,respectively. In case the wetness period was more than 5 days, the typical symptom was not found on pepperfruits because of the overgrowth of mycelia. Using this fruit assay method, which the pepper anthracnosepathogens were inoculated by spraying spore suspension on non-wounded or wounded pepper fruits, controleffect of three fungicides were evaluated against pepper anthracnose by the protective and/or the curativeapplication. Propineb showed high protective control activity, while it showed curative control activity on un-wounded fruits, but did not showed curative control activity on wounded fruits. Tebuconazole, one of curativefungicide, showed higher control activity in non-wound inoculation than wound inoculation. Trifloxystrobin,one of strobilurin group, showed high both protective and control activity against anthracnose. In conclusion,we supposed that the newly developed in vitro pepper fruit assay can be used to evaluate antifungal activityof control agents against pepper anthracnose.Key words Colletotrichum acutatum, fungicide activity, in vitro fruit assay, pepper anthracnose