Риск патозооспермии у мужчин при совместном воздействии эндокринных дизрапторов

Abstract

84 samples of sperm were studied in men with normo-and patozoospermia. In seminal fluid, the concentrations of bisphenol A, triclosan and 4-nonylphenol were simultaneously determined by gas chromatography with mass spectrometry (GC-MS). The spermiological study was carried out according to the WHO recommendations (2010) taking into account the assessment of the number of spermatozoa, their motility and morphology, as well as sperm damage. The results were subjected to statistical processing using the Mann-Whitney U-test and the multiple logistic regression method. Results were considered statistically significant at p<0.05. Bisphenol A was found in 100% of the ejaculate samples with a median concentration of 0.150 ng/ml. Triclosan and 4-nonylphenol were detected in 84.3% and 98.1% of ejaculate samples with a median concentration of 0.11 ng/ml. and 0.16 ng/ml, respectively. The comparison groups were statistically significantly distinguished by the concentrations of bisphenol A and triclosan (p<0.001; p=0.018), respectively, as well as the DNA index of spermatozoa fragmentation (p=0.002). Bisphenol A, by increasing the concentration in seminal fluid by 0,1 ng/ml, increased the chance of developing pathosospermia by 24.9 times. The effect of triclosan and 4-nonylphenol on pathosospermia was not statistically significant. In a multiple logistic regression equation describing the joint effect of EDs on patozoospermia, as well as the ratio of the chances of its occurrence with a change in the concentration of one of the endocrine disruptors per unit while fixing the other variables, the coefficients of triclosan and 4-nonylphenol on the patozoospermia were also not statistically significant. In addition, the confidence interval for the odds ratio included one. The development of pathozoospermia is primarily associated with the effect of bisphenolA in seminal fluid in men. It should be assumed that there is no synergistic effect of endocrine disruptors on patozoospermia.