Abstract

Feline calicivirus (FCV), as well as the bacteria Bordetella bronchiseptica, Chlamydia felis and Mycoplasma felis are the top five most common pathogens that cause URTI. A Kennel Cough or Infectious Respiratory Disease in Dogs (IRS) has a complex etiological cause.
 
 The purpose of this study is to analyze the prevalence of the main pathogens that cause URTI and IRS entering the veterinary clinics of Russia. By real time PCR (profile) from material obtained from 5 520 cats for 5 infectious agents, 81.7 % were positively detected, and in 25.9 % of cases with the appearance of co-infections. The DNA of the bacterium B. bronchiseptica was detected 3.7 % (n = 233), C. felis 11.2 % (n = 698), M. felis 21.5 % (n = 1347). FCV RNA was detected in real time reverse transcription PCR in 21.4 % of cases (n = 1340), FHV-1 DNA in 16.3 % (n = 1017) of samples. When examining 1742 dogs for six causative agents of the IRRS: parainfluenza viruses (CPiV), distemper (CDV), adenovirus type 2 (CAV-2), canine herpesvirus (CHV), B. bronchiseptica, Mycoplasma cynos ― positive 981 were detected samples (56.3 %), the only pathogen was detected in 740 cases (75.4 %). In 206 cases, B.bronchiseptica DNA was detected, in 13 CAV-2, in 272 CPiV, and in 84 CDV RNA, CHV ― 17, M.cynos ― 389. Thus, the proportion of positive reactions arriving at B. bronchiseptica was 15.9% , for CAV-2 ― 1.01 %, for cases of CPiV ― 20.8 %, and for CDV ― 6.4 %, for M. cynos29.8 %, CHV 1.3 %, for mix infection ― 24.6 %.

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