Пренатальное влияние марганца на уровень сывороточной ацетилхолинэстеразы у крыс

Abstract

The prenatal period is critical to the development of the nervous system. In spite of the fact that manganese is an essential element, excessive exposure to this metal can lead to negative postnatal consequences. This paper examined the prenatal effect of manganese sulphate on acetylcholinesterase (AChE) concentration in two generations of rats. For that purpose, female Wistar rats received a basic diet with additional MnSO4 (1433 mg/kg/day) for 28 days before gestation and during gestation (20–26 days). Young offspring of the first generation were tested for behavioural disorders using standardized behavioural assays, and, as a result, male rats with most abnormalities were selected. Firstgeneration female offspring were subsequently used to obtain second-generation offspring, from which males with behavioural disorders were also selected. Blood samples were taken from the selected animals at the age of 18 weeks to determine AChE level. The effect of oral exposure to manganese on the body of mother rats was also assessed. The study found that female rats directly exposed to MnSO4 tended to have higher AChE levels. Statistically significant changes in the level of this enzyme were observed in first-generation animals: their first quartile was 22 % greater than the third quartile of the group that had not been prenatally exposed (p = 0.012). Second-generation animals tended to a have higher blood AChE concentration compared to intact animals. Thus, the research demonstrated that prenatal exposure to manganese sulphate affects AChE level both in the maternal body and in several generations of offspring. For citation: Notova S.V., Karimov I.F., Kazakova T.V., Marshinskaya O.V. Prenatal Effect of Manganese on the Serum Level of Acetylcholinesterase in Rats. Journal of Medical and Biological Research, 2021, vol. 9, no. 2, pp. 163–170. DOI: 10.37482/2687-1419-Z054