Abstract
The goal of this study was to investigate the extracts of ellagic acid in Korean and Chinese black raspberries according to harvest time and extraction solvent. This study was also performed to establish a simple and reliable high-performance liquid chromatography-ultraviolet (HPLC/UV) analytical method for the determination of ellagic acid for quality control and to establish baseline data of extracts of Rubus occidentalis (uRo) and Rubus chingii (uRc) fruits as healthy functional food ingredients. uRo and uRc were each extracted with 0%, 5%, 30%, and 70% ethanol. Among the extracts of these two varieties, the 5% ethanol extracts of uRo and uRc showed the highest contents of ellagic acid, 16.56 and 17.27 mg/g, respectively. The specificity was satisfied with retention time and photo-diode array (PDA) spectrum by analysis of ellagic acid using HPLC and comparison with a standard compound. Ellagic acid was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. It showed a high linearity in the calibration curve, with a coefficient of determination (R2) of 0.9999, and the LOD and LOQ values were 0.25 and 0.77 μg/mL, respectively. The results showed that R. occidentalis and R. chingii can be used simultaneously as healthy functional ingredients and natural medicines.
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