Вплив фетоплацентарної недостатності матерів на стан сперматогенезу самців нащадків

Abstract

The demographic situation in most countries of the European region, which includes Ukraine, is characterized as quite complex. Nowadays the question of the long-term effects of maternal fetoplacental insufficiency on the functioning of human body systems, in particular on the reproductive system of male offspring, remains open. It is known that negative factors during pregnancy can affect the development and existence of the individual. The purpose of the work was to study the long-term effects of fetoplacental insufficiency on the functioning of the reproductive system of adult male offspring born by mothers of different ages. Material and methods. The study was performed on healthy adult female Wistar rats, young (3 months) and mature (10 months) of reproductive age. 4 groups were formed: the 1st and the 2nd groups included intact animals of young and mature age; the 3rd and the 4th had females with experimental fetoplacental insufficiency of young and mature reproductive age. Modeling of fetoplacental insufficiency was performed by daily subcutaneous injection to females from the 12th to the 18th day of pregnancy 50% oil solution of carbon tetrachloride at a dose of 2 ml/kg body weight. We studied the state of spermatogenesis, weight of internal organs, sex hormones in mature male offspring of 3 months of age after decapitation. Results and discussion. Fetoplacental insufficiency leads to lower testosterone levels in all offspring born to mothers of different reproductive ages. The total level of estradiol remained almost unchanged, but still, in animals there was a shift in the ratio of sex hormones in the direction of hyperestrogenism. Experimental fetoplacental insufficiency in females of different reproductive ages also affected the mass of the testes, epididymis and adrenal glands in their male offspring. According to the indicators of the functional state of epididymal sperm in animals born by reproductively young females, the share of pathological forms of sperm decreased by 45%, in the offspring born by reproductively mature females with fetoplacental insufficiency decreased the number of motile sperm by 46% compared to the intact group of animals. Conclusion. The penetration of toxic substances into the mother's body leads to varying degrees of total xenobiotic load, followed by induction of neutralization reactions and the development of metabolic forms of fetoplacental insufficiency, changing the function of the endocrine system and causing adverse effects on the reproductive system. Fetoplacental insufficiency affects the reproductive function of adult male offspring born to mothers of different reproductive ages, which is manifested in a decrease in testosterone levels and deterioration of the spermogram, which in turn can lead to problems with impregnation