Abstract
Endothelial cell growth factor from platelets, designated as Vascular Endothelial cell Proliferation Factor (VEPF), was purified to an apparent homogeneity from fresh human platelets using eight steps, e. g.: QAE-Sephadex chromatography, ammonium sulfate precipitation, DEAE-Sepharose chromatography, chromatofocusing separation, Mono P chromatography and HPLC gel chromatography using Superose 12 and TSK 4000 columns. The half maximal stimulation was obtained at a concentration of less than 10ng/ml (less than 200pM). The highly purified material stimulated the growth of porcine aortic endothelial cells but was inactive against human foreskin fibroblasts.
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