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Abstract
The medium used for microbial fermentation is generally in a liquid state, that is, a fluid. Here, there is no fixed shape, and the flow is irregular. Attempts have been made to analyze and predict the flow of the fluid. Kluyveromyces marxianus JMA-1 was developed to produce 3-hydroxy propionic acid (3-HP), a biopolymer precursor, and the agitation conditions were optimized by applying hydrodynamics. The maximum flow velocity and Γ1 value indicating the degree of rotation were the highest (45.35 ㎝/s and 0.72, respectively) in the control (Baffle 0) and the lowest (20.99 ㎝/s and 0.40, respectively) in Baffle 2. However, 3-HP production was the highest (8.82 ± 0.24 g/L) in Baffle 2 and the lowest (6.83 ± 0.11 g/L) in the control. The 3-HP production of Baffle 2 improved by 29% compared to that of the control.
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