МЕТОДЫ ИДЕНТИФИКАЦИИ КАРБОНОВЫХ КИСЛОТ В СОСТАВЕ ЭКЗОМЕТАБОЛИТА ПРОБИОТИЧЕСКОЙ КУЛЬТУРЫ LACTOBACILUS PLANTARUM 8P-А3

Abstract

Abstract. Human life in modern conditions is characterized by the constant influence of adverse environmental factors affecting the neuropsychological, physiological and metabolic parameters of the body. The compensatory capabilities of each individual, including the immune state, are largely determined by the state of the microbiota. In practical microbiology, research is relevant in the development of a new generation of drugs designed to maintain and restore normal flora, including fundamentally new probiotics - metabiotics created on the basis of microbial exometabolites. Taking into account the demand for metabiotics in the pharmaceutical market, an exometabolite complex was proposed for the design of such preparations, obtained by ultrafiltration of the culture liquid of a probiotic strain used in the production of lactobacterin. It is known that, along with bacteriocins, carboxylic acids remain among very significant components of the metabolite complexes of lactobacilli. In this regard, the purpose of this work was to choose the optimal physicochemical method for the joint determination of carboxylic acids on the model of the complex of exometabolites of the probiotic culture Lactobacilus plantarum 8P-A3.As a result of the studies, it was found that the optimal physicochemical method for identifying carboxylic acids in the composition of the metabolite complex is the method of capillary zone electrophoresis. In the composition of metabolites, such significant acids as lactic (217.7 g/l), citric and acetic (29.6 and 10.2 g/l) were identified. The simplicity and expressiveness of this method, combined with its high sensitivity in the determination of carboxylic acids, including in samples with complex matrices, is noted.