ВОЗМОЖНОСТИ ИСПОЛЬЗОВАНИЯ НАНОНИТЕЙ НА ОСНОВЕ ПОЛИВИНИЛПИРРОЛИДОНА ДЛЯ ИММОБИЛИЗАЦИИ КЛЕТОК LACTOBACILLUS ACIDOPHILUS

Abstract

This study investigates the possibility of using polyvinylpyrrolidone (PVP) nanofilaments for the immobilization of Lactobacillus acidophilus strain Ep 317/402 n. v. Narine AAA. Two types of nanofilaments - PVPI and PVPII - with a diameter of 200-300 nm obtained by the electrospinning method were experimentally tested. The former type consisted in pure high-molecular polyvinylpyrrolidone nanofilaments, whereas the latter additionally included zinc oxide. The electrospinning unit consisted of a NE-300 syringe pump and a high-voltage INVER-30/5 source for producing an electric field of 1.8 kV/cm. The effectiveness of the immobilization using polyvinylpyrrolidone was evaluated according to the condition and morphology of Acidophilic Lactobacilli cells, their growth rate and the culture medium pH. The PVP modification with zinc oxide is shown to stimulate the acid resistance of probiotic bacteria and to cause the formation of a stable biofilm from exogenous metabolites. Scanning electron microscopy was used to establish the heterogeneous character of the exometabolic matrix, which predominantly consists of extracellular fibres temporarily held on the bacterial cell surface. The obtained results confirm the prospects of using PVP nanofilaments and their modifications for the optimization of probiotic immobilization technologies.