РАЗРАБОТКА УСКОРЕННОЙ СХЕМЫ ИДЕНТИФИКАЦИИ ЛИСТЕРИЙ С ПОМОЩЬЮ ФАГОВОГО БИОПРЕПАРАТА L. M 4 УЛГАУ

Abstract

The causative agent Listeria monocytogenes causes listeriosis, a severe foodborne illness associated with high mortality. Rapid and sensitive methods are required to detect and identify this pathogen in the food production process. The article presents the results of research on the development of technological parameters for the production of biopreparation based on the bacteriophage L. m 4 UlSAU for quick identification of bacteria of the genus Listeria with its help. It was established that for the production of L. m 4 phage of UlSAU with maximum titers, the optimal parameters are: multiplicity of MOI 1 infection, cultivation temperature-28°C, incubation time of the phage/ culture system – 6 hours. Based on these results, we have offered technological scheme for the production of phage biopreparation L. m 4 UlSAU, which includes the following steps: confirmation of biological properties of indicator phage and increasing its titer (if necessary), verification and confirmation of biological properties of indicator culture, production of phage biopreparation and control of its indicators. Accelerated scheme for Listeria identification was offered with the help of prepared biological product based on L. m 4 bacteriophage of UlSAU. The scheme was tested on samples of chicken meat and minced meat semi finished products artificially contaminated with Listeria monocytogenes bacteria in concentrations 101-105 CFU/ml. It was established that the proposed scheme of listeria identification allows to reduce the duration of studies by 84 hours in comparison with the traditional bacteriological method and to detect listeria at a concentration of 100 CFU/ml (g) in 82 hours. The phagoidentification time at the pure culture typing stage is 18 hours.