영여자 절편체 배양 및 액체배지 첨가에 의한 둥근마의 기내 대량번식

Abstract

Shoot tips of chinese yam (Dioscorea opposita Thunb) were cultured on MS medium containing 0.5 ㎎/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 ㎎/L BA and 0.5~1.0 ㎎/L IAA. Sucrose added to MS medium with 2.0 ㎎/L BA and 0.5 ㎎/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks` culture of micro-tuber sections on the medium with 2.0㎎/L BA, 0.5㎎/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25㎖ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.