ЭПИЗООТОЛОГИЧЕСКИЕ ОСОБЕННОСТИ И ЛАБОРАТОРНАЯ ДИАГНОСТИКА КАМПИЛОБАКТЕРИОЗА ПРОДУКТИВНЫХ ЖИВОТНЫХ И ПТИЦЫ

Abstract

Campylobacteriosis is a zoonotic infectious disease of animals and humans, characterized by various degrees of severity and polymorphism of manifestations: reproductive pathology, damage to the gastrointestinal tract, in humans -toxicoinfection. The aim of the work is to identify the epizootological and epidemiological role of certain species and subspecies of pathogenic campylobacteria in the infectious process in productive animals. The work was carried out for 12 years in livestock and poultry farms on the territory of the Northwestern Federal District of the Russian Federation. The diagnosis was established on the basis of complex epizootological, bacterioscopic, bacteriological, serological, molecular biological studies. Season prevalence of the disease in animals husbandry wasn’t been established and poultry revealed such prevalence. During the bacteriological study of 4163 mixed cultures from cattle, sheep, pigs, poultry, 67.59% of pure campylobacter cultures were isolated. The maximum specific part of campylobacter host, on average, was detected in poultry —79.49% and cattle — 71.79%; in pigs — 63.62%, in sheep — 52.61%, respectively. The circulation of two pathogenic subspecies of campylobacteria in the infectious process that cause epidemiological risks in animals has been established: C. jejuni sbsp. jejuni with the maximum level in poultry — 79.03 % and C. fetus sbsp. fetus, respectively, in sheep — 46.79 %. PCR using the russian test system СAM—BAC revealed a high specificity for the DNA of campylobacteria from cattle of the subspecies C. jejuni sbsp. jejuni. It is advisable to create a database on pathogenic campylobacteria and exchange information between veterinary and medical services for surveillance of this zoonosis.