ВЛИЯНИЕ СТРОНГИЛОИДОЗНОЙ ИНВАЗИИ ЖЕРЕБЯТ НА НЕКОТОРЫЕ ПОКАЗАТЕЛИ ФУНКЦИОНАЛЬНОЙ АКТИВНОСТИ ПЕЧЕНИ

Abstract

The diagnosis of strongyloidiasis in foals was established on the basis of clinical signs and the results of copro- and larvoscopic studies using the Darling and Berman-Orlov methods (in the modification of Shcherbovich). For 12 months, blood samples were taken from foals of both groups for biochemical analysis. Studies of the amount of total protein in blood serum were carried out by the biuret method, the amount of urea - by reaction with diacetyl monooxime, uric acid - by reaction with a phosphotungstic reagent (Muller-Seifert method), glucose - by the enzymatic method (enzymatic method with glucose oxidase), the amount of total bilirubin - with the diazonium salt of sulfanilic acid, determination of the activity of blood serum enzymes (AST, ALT, alkaline phosphatase and LDH) was carried out using kinetic methods based on the recommendations of the International Clinical Federation (IFCC), the ratio of protein fractions (albumin) - by electrophoretic separation of proteins on agarose for determination of indicators of the functional activity of the liver in foals was formed according to the principle of conditional analogues 2 groups of one-month-old animals: experimental - foals spontaneously infected with strongyloidiasis; control - clinically healthy foals. Monthly during the year, blood samples were taken from them, in which the content of total protein, albumin, total bilirubin, urea, uric acid, glucose, as well as the activity of aspartate and alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase were determined. The infested foals in the blood serum showed a decrease in the amount of total protein and albumin fraction, high levels of bilirubin, urea, uric acid, glucose, and an increase in the activity of AST, ALT, alkaline phosphatase and LDH. Changes in the studied parameters persisted throughout the entire period of research, but the most significant deviations were noted in foals at the age of seven and eight months. Deviations from the norm in the main indicators characterizing the functional activity of the liver in foals of the experimental group indicate damage to hepatocytes, a violation of the synthetic and secretory functions of the liver.