추출 온도에 따른 산수유의 항산화 활성 및 항유전독성 효과 비교

Abstract

The objective of this study was to evaluate the antioxidant and antigenotoxic activities of sansuyu fruit (Corni fructus, CF) at temperatures of 25℃, 50℃, and 90℃ using a water extraction method. Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity, and ORAC (Oxygen radical absorbance capacity) values were determined. Also the antigenotoxicity of CF was determined by measuring inhibitory effects of H₂O₂ induced DNA damage in human leukocytes using the comet assay. The TPC in the CF extracts was 4.2, 4.6, and 5.5 g/100 g GAE in 25℃, 50℃, and 90℃, respectively. The DPPH RSA of the CF extracts increased in a dose-dependent manner over the range of 50~1000 μg/㎖ in all temperatures and the SC?? of DPPH RSA of the CF extracts were not significantly different at different extraction temperatures. The SC50 of SOD-like was the highest in CF extracted at 25℃ (1.1 ㎎/㎖) followed by 90℃ (1.2 ㎎/㎖) and 50℃ (1.3 ㎎/㎖). The ORAC values of the CF extracts were not significantly different in low concentration (10 μM/㎖) and was in order of 25℃ (5.7 μM TE)＜90℃ (6.2 μM TE)＜ 50℃ (8.5 μM TE) in high concentration (50 μM/㎖). 200 μM H2O2 induced DNA damages in human leukocytes were significantly reduced by the pretreatment with the CF extracts. These results suggest that sansuyu fruit (Corni fructus) can be used as a natural source for antioxidant activities and as antigenotoxic agents regardless of the water extraction temperature.