ИММУНОФЕНОТИПИЧЕСКАЯ ХАРАКТЕРИСТИКА ПРОЛИФЕРАЦИИ И АПОПТОЗА ИНТАКТНЫХ КЕРАТИНОЦИТОВ ПРИ СИСТЕМНОМ ВОСПАЛЕНИИ

Abstract

In response to infection by pathogenic microbes, a serious systemic inflammation occurs in the body, often lead- ing to death. Unbalanced systemic immune responses can lead to the accumulation of leukocytes, disseminated intravascular coagulation and microcirculatory dysfunction, followed by apoptosis and cell necrosis, as well as the development of multiple organ failure syndrome. The destruction of the barrier can apparently lead to a violation of the proliferation and apoptosis of keratinocytes. The aim of the study was to evaluate intracellular protein-regulators of the life cycle of keratinocytes of the intact epidermis in case of systemic inflammation. Material and methods. The study was performed on 40 archival paraffin blocks of skin fragments in two groups: group I (n = 30) - deceased patients with a diagnosis of severe bacterial sepsis; Group II (n = 10) – dead for reasons not related to an infectious disease (control). Immunohistochemical studies were performed according to a standard protocol using antibodies to Ki-67, caspase 3, Bcl-2 and p53. Results. In the cells of the epidermis of patients in the condition of systemic inflammation, the following expression of the studied markers is observed: caspase-3 – 39.4 ± 1.2%; p53 – 18.4 ± 0.7%; Ki-67 – 15.2 ± 4.1%; Bcl-2 – 4.2 ± 1.2%. Conclusion Under conditions of systemic inflammation in the epidermis, there is an imbalance in the proliferative- apoptotic activity of keratinocytes in the direction of reducing their mitotic division and activation of apoptosis, leading to cell death.