Abstract
Introduction. The development of methods for determining the concentration of antimicrobial drugs used in various pathological conditions of the body is of great interest. Aim. Development of a technique for quantitative determination of amphotericin B in blood serum. Materials and methods. The drug amphotericin B was used for the preparation of solutions. The optical density of the solutions was determined by the spectrophotometric method. Results. The method of quantitative determination of amphotericin B in sodium chloride solution, which is the closest in composition to biological fluids, has been tested. Statistical analysis showed that the error of the method for determining the concentration of amphotericin B does not exceed 10%. Conclusion. A unified method of spectrophotometric determination of amphotericin B in blood serum has been developed. The optimal conditions for the determination of amphotericin in biological fluids were found: the solvent – 0.9% sodium chloride solution, wavelengths – 363, 383 and 406 nm, t – 3–6°C, detectable concentrations – from 1.0 · 10–2 to 1.0 · 10–5 mg/l.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
