Abstract

The article is devoted to the development of technology and equipment for the production of bioethanol from agricultural plant waste, activated by the steam explosion method. The value and novelty of research lies in obtaining new data on the effective acidic and enzymatic hydrolysis of activated raw materials, and developing a technology for the conversion of plant raw materials into bioethanol. The studies were carried out on the basis of the Department of Wood Materials Processing of Kazan National Research Technological University (Republic of Tatarstan, Kazan). A pilot plant for the production of bioethanol and the principle of its operation are presented. Pine wood waste and wheat straw (collected in Kukmor region of the Republic of Tatarstan in the period August-September 2021) were used as raw materials. Steam-explosive activation of raw materials was carried out at temperatures of 165 ⁰C and 210 ⁰C for 5 minutes. Acid hydrolysis parameters: H2SO4 concentration - 0.5% and 1.5%, hydromodule 1:15, hydrolysis temperature - 187⁰C, hydrolysis duration - 5 hours. Enzymatic hydrolysis parameters: preparation - Cellulox-A (OOO PO Sibbiopharm, Russia) - 6 and 12 g/kg of raw material, hydrolysis temperature - 45 ⁰C, substrate pH 4.7 (acetate buffer), raw material concentration in the substrate 33 g/l, the duration of hydrolysis is 72 h. Alcoholic fermentation of hydrolysates was carried out at 32-34⁰C using Saccharomyces cerevisiae yeast, fermentation duration 7 h, yeast concentration 25 g/l. The bioethanol yield in % of reducing substances was recalculated after determining the mass yield. It is concluded that the vapor-explosive activation of pine wood at a temperature of 210 ºC makes it possible to obtain by acid hydrolysis and anaerobic fermentation of reducing substances up to 0.26 kg (0.33 l) of ethanol from 1 kg of activated raw materials, and activation of wheat straw at the same temperature allows obtaining up to 0.172 kg (0.218 l) ethanol with 1 kg of activated straw

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