Abstract
With the increasing number of women-participants in piloted space programs, investigations of spaceflight effects on the female reproductive system are becoming vital. Purpose of the work is to determine thickness of the granulosa cells layer relative to the content of the luteinizing hormone (LH) receptor in antral follicles of the ovary, LH and a relative content of cytoskeletal proteins in the hypophysis of mice following suspension during the estrous cycle. SDS-PAGE and immunoblotting were used to define the relative proteins content. mRNAs were counted using RT-PCR. IHA was performed to measure thickness of the granulosa cells layer in ovarian sections. The results demonstrate an increase in the number of granulosa cells in the antral follicles of mice after 96-hr suspension, as well as in the relative content of the LH receptor and its mRNAs. However, the LH level in the hypophysis did not alter, even though we observed some changes in the content of cytoskeletal proteins, namely, an increase in the actin-binding proteins (alpha actinin 1 and 4) and a decrease in alpha tubulin and its acetylated form suggesting, probably, modification of the intracellular LH transport and release into blood.
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