전사인자 발현 조절을 통한 황금/아티초코 복합추출물의 지방분화 억제 효과

Abstract

In this study, we investigated cytotoxicity, inhibition of adipocyte differentiation, lipolysis, and expression of obesity-related transcription factors inhibition of the Scutellaria baicalensis Georgi/Cynara scolymus L. mixture extract (SCME)to evaluate its use as an anti-obesity natural material. As a result, cell viability for 3T3-L1 cells did not affect cell growth up to a concentration of 500 μg/ml in all samples. Adipocyte differentiation and fat accumulation inhibited in a concentration-dependent manner by Scutellaria baicalensis Georgi extract (SGE) and Cynara scolymus L. leaf extract (CLLE) treatment. In the case of adipocyte differentiation, SCME suppressed more than the SGE and CLLE at a concentration of 500 μg/ml. The amount of accumulated fat was also lower in SCME (32.2%) than in AGE and CLLE (47.7~41.0%). Lipolytic efficacy was reduced by 48.2% compared to the control when SCME was treated at a concentration of 500 μg/ml. Expressions of PPAR-γ, aP2, and ADIPOQ were also decreased in a concentration-dependent manner by SCME, and were decreased by 19.7%, 42.0%, and 12.7% at a concentration of 1,000 μg/ml, respectively. As a result of the above, it is judged that SCME exhibits effects of suppressing adipocyte differentiation and fat accumulation and lipolysis by regulating the expression of transcription factors related to adipocyte differentiation and fat accumulation. In order to utilize SCME as an anti-obesity material, Future study is necessary to conduct preclinical experiments in the future.