ЭКСПЕРИМЕНТАЛЬНО КЛИНИЧЕСКОЕ СОПОСТАВЛЕНИЕ АЛКОГОЛЬ-ИНДУЦИРОВАННЫХ ИЗМЕНЕНИЙ ВАСКУЛЯРНО-ЭНДОТЕЛИАЛЬНОГО ФАКТОРА РОСТА

Abstract

Aim: to comparatively estimate the nature and direction of alcohol-induced changes of vascular endothelial growth factor in the experiment and in the clinic. Materials and methods. The experimental part of the research was performed on 50 outbred mature rats (15 females and 15 males exposed to alcohol intoxication for 30 days, and 10 females and 10 males formed a control group). The average age of males was 38 (37.25–39) weeks, females - 40.5 (40-42.5) weeks. The body weight of females was 340 (329.5–345.5) g, males – 469 (464.5–474.25) g. After fixation, the control group animals were injected with intragastrically sterile 0.9% aqueous sodium chloride solution, the rest of the animals – 40% ethanol daily at the rate of 10 ml / kg, so that the animals reached a mild degree of intoxication. Clinical part of the research included 60 young patients: 30 males and 30 females, mean age was 35,97 (34,00-40,00) in females and 37,5 (34,00-41,00) in males, who were admitted to the narcological hospital for treatment of intoxication with diagnosed alcohol use disorder. The serum concentration of VEGF-A was evaluated in the blood serum of the studied patients and animals. Results. Alcohol intoxication caused an increase of serum concentration of VEGF-A in both animals and patients in comparison with control groups. The changes were unidirectional with no significant gender differences. Conclusion. The analysis of the experimental-clinical comparison of alcohol-induced changes in the serum content of VEGF-A indicates the unidirectional nature of the biological (angiogenic) effect in the form of VEGF-A expression under the action of ethanol. In the clinical interpretation of the physiological or pathological expression of angiogenesis, accompanied by an increase in the serum concentration of VEGF-A, it is necessary to take into account the specifics of the pathological process.