Abstract
Objectives: The purpose of this study was to provide the importance of surface management through the identification of bacteria detected on the surface of a computer mouse attached to a dental unit chair.
 Methods: After rubbing with a sterilized cotton swab in the center of the left mouse button in the range of 1 cm × 1 cm, it was immediately put into 1.5 mL of sterile saline. colony forming units (CFUs) grown on Lysogeny broth (LB) agar plates were analyzed through Polymerase Chain Reaction (PCR).
 Results: About 8 types of bacteria, gram-negative and gram-positive, were detected on the surface of the computer mouse attached to the dental unit chair.
 Conclusions: Infection control in the dental office should be prevented through surface contamination management of computer mouse attached to dental unit chairs.
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