Abstract
The effect of the neuropeptide dalargin, which is a synthetic analogue of leu-enkephalins, on the negative processes caused by cold stress, which occur when artificial hypothermia is used in vivo, was studyd. It was shown that dalargin prevents the development of apoptotic processes in the cells of homeothermic animals after cold stress, which manifested itself in a decrease in the degree of DNA fragmentation. In the kidneys and liver of experimental animals subjected to cooling, this attribute of apoptosis and cell necrosis was not detected, and in the intestinal mucosa and spleen the level of DNA fragmentation as a result of cold stress is statistically significant. The preliminary injection of dalargin to rats reduces the degree of DNA fragmentation to values that occurred in animals that were not exposed to cold stress. It is also shown that dalargin prevents the development of adrenal hypertrophy in experimental animals, reduces the degree of involution of the thymus and spleen, and reduces the severity of cold stress-induced ulcerogenesis. The simultaneous injection to animals of an opiate receptor antagonist naloxone eliminated the protective effect of an opiate receptor agonist dalargin.Probl Cryobiol Cryomed 2019; 29(3): 246–254.
Highlights
The effect of neuropeptide dalargin, that is synthetic analogue of leu-enkephalins, on the cold stress-induced negative processes, occurring under artificial hypothermia in vivo, has been studied
It is known that in homeotherms and human, a number of processes are triggered in cells, tissues, certain organs and at the whole body level due to a significant temperature decrease relative to physiological norm [5, 6, 18], i. e. there is a decrease in the intensity of metabolic process and immunological reactivity of a body, the reduction of oxygen consumption, accompanied by hypoxia development, and CNS function inhibition
Our previous detailed analysis of findings of cooling impact on cell apoptosis [4] shows its signs as manifested not during cell staying in hypothermic medium, but after their return under normothermia and further exposure within a certain time period
Summary
The effect of neuropeptide dalargin, that is synthetic analogue of leu-enkephalins, on the cold stress-induced negative processes, occurring under artificial hypothermia in vivo, has been studied. The dalargin was demonstrated to prevent an apoptotic process development in cells of homeotherms after cold stress, that was manifested in a decreased level of DNA fragmentation. E. there is a decrease in the intensity of metabolic process and immunological reactivity of a body, the reduction of oxygen consumption, accompanied by hypoxia development, and CNS function inhibition.
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