Abstract
Methods of multicomponent spectral analysis of exhaled air based on application of tunable diode lases were proposed for biomedical diagnostics. Simmultaneous laser based spectral analysis of the gas pairs like CO and CO2, and CO and N2O in the 4.7 micron spectral region could be used for investigation of gas exchange in studies on breathing phisiology and cardiovascular diagnostics conducted with application of different loading tests, as well as for studies in anesteziology and for on-line monitoring of ventilation-perfusion ratio. Simmultaneous analysis of NO and CO2 in 5.4 micron region could be useful fot the control of breating maneuver in studies of inflammation processes in the distal part of the lungs. Simmultaneous detection of NH3, СO2 and C2H4 near 10.5 m could be promising in studies of basal metabolizm and metabolis cycles. Simmultaneous analisis of 13СO2 and 12С O2 near 2.05 m could be used for the detection of 13СO2/12СO2 izotope ratio for izotope breath tests. The results of promising spectral region analysis and of mutual location of absorption analytical lines in these regions are presented. A possibility of simmultaneous detection of several studied molecules in the provided spectral regions was experimetally demonstrated as well as experimental laser spectra of molecular absorption were obtained. A possibility of the proposed approach in applications to analysis of trace gases in exhaled air was demonstrated.
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