ГЕМОСТАЗКОНТРОЛИРУЮЩАЯ АКТИВНОСТЬ И ВОДНЫЙ БАЛАНС ЛЕГКИХ В ДИНАМИКЕ ЭКСПЕРИМЕНТАЛЬНОЙ ИШЕМИИ МОЗГА

Abstract

The study aims to investigate the system of hemostasis and fibrinolysis in venous and arterial blood, the features of blood filling and lung water balance, as well as the activity of lipid peroxidation (LPO) processes in the lung tissue iduring the acute cerebral ischemia. Material and methods. The experiments were performed on non-pedigree male rats, including control and sham operated ones. Acute cerebral ischemia was modeled by the bilateral occlusion of the common carotid arteries. Survived animals were assessed according to the degree of neurological deficit using the McGraw scale. Complex studies included determination of activated partial thromboplastin time, prothrombin time, time of XIIa-dependent fibrinolysis of arterial and venous blood, LPO intensity by the content of thiobarbituric acid active products and catalase activity in the lung tissue, the number of circulating endothelial cells; assessment of the lung blood filling and water balance by the gravimetric method. Results. In 24 hours after cerebral ischemia we found that the coagulation potential of blood increased in the systemic circulation and the pulmonary circulation affected by inhibition of the fibrinolysis system. On the 21st day of cerebral ischemia, dysregulation of hemostasis mechanisms in the pulmonary bloodstream was revealed in the form of hypercoagulation of blood and hyperfibrinolysis. At the same time the increase of malondialdehyde, blood circulation, and the content of the extravascular fluid in the lungs were noted. The correlation between the intensification of LPO processes with an imbalance of pro- and anticoagulants and hyperhydration of the lung tissue has been shown. The obtained results indicate a progressive course of disorders of the non-gas exchange functions of the lungs in the dynamics of acute experimental cerebral ischemia.