Abstract
Abstract-Lipids make up more than half of the dry matter of the human brain and play a key role in its functioning. However, the lipid composition of the brain anatomical structures remains poorly understood. The first such studies were carried out more than 50 years ago, but since then, a small number of works have been published describing the concentration of lipids in only a few areas of the human brain. A fundamentally new step towards understanding the molecular organization of the brain and identifying the molecular basis of human cognitive abilities should be a detailed large-scale study of the brain lipidome. However, there is no description in the literature of methods optimized for studying the lipid composition of the human brain. In this work, we develop and present methods for lipid extraction and mass spectrometry analysis, which ensure simultaneous detection of the maximum amount of different lipid classes and individual substances in the human brain, as well as the approaches to bioinformatics analysis of the obtained data. Their use makes it possible to create a comprehensive picture of the molecular organization of the human brain, which has no analogues in the world in terms of its completeness. Key words: lipidome, brain, mass spectrometry, high-performance liquid chromatography, bioinformatics The reported study was funded by the Russian Foundation for Basic Research, project no. 20-34-70077.
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