ПУТИ РЕАЛИЗАЦИИ АПОПТОЗА ЛИМФОЦИТОВ ЧЕЛОВЕКА, ИНДУЦИРОВАННОГО ПЕРОКСИДОМ ВОДОРОДА, И ИХ РЕГУЛИРОВАНИЕ

Abstract

The changes in marker indicators of apoptotic cell death were studied: the level of functional activity of initiating caspases-8, -9, -12, membrane death receptors Fas, intracellular reactive oxygen species, cytosolic calcium, mitochondrial potential of peripheral blood lymphocytes of donors induced by exposure to hydrogen peroxide at final concentrations of 10-6 and 10-5 mol/l. Activation of initiating caspase-8 associated with membrane death receptors Fas was detected after exposure to hydrogen peroxide lymphocytes at a concentration of 10-6 mol/l. There was a significant increase in the functional activity of initiating caspase-9, the level of Fas receptors and intracellular reactive oxygen species, as well as a decrease in the concentration of cytosolic calcium and the value of the mitochondrial membrane potential 1 and 2 hours after modification of cells with hydrogen peroxide at a concentration of 10-5 mol/l compared with those for intact immunocytes. It was found that apoptosis of lymphocytes under the influence of exogenous hydrogen peroxide is realized with the participation of receptor-mediated caspase, mitochondrial (with activation of caspase-9) and p53-dependent mechanisms. The protective effect of resveratrol (10-6 mol/l) on lymphocytes after exposure to hydrogen peroxide (10-5 mol / l), which is due to its ability to reduce the level of reactive oxygen species in intact and modified hydrogen peroxide immunocytes, as well as deactivate hydrogen peroxide.