Гистологическое исследование нативных и криоконсервированных неонатальных семенников крыс после интратестикулярной аллотрансплантации

Bo Deng,Vitaliy Kireev,Kristina Melentyeva,Oleksandr Pakhomov,Igor Kovalenko

doi:10.15407/cryo30.01.034

Abstract

Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. Moreover, the transplantation of testicular tissue is involved into investigation of the inborn and acquired defects of testicular tissue. Thus the improvement of cryopreservation and transplantation methods is of great interest. Here, we have compared the findings of histological examination of allotransplanted native and cryopreserved neonatal testis. It has been shown that the usage of DMEM/F12 supplemented with 5 mg/ml bovine serum albumin, 0.05 M sucrose, 20 mM Hepes, 1.5 M dimethylsulfoxide as a cryoprotectant, controlled cooling, preservation at -196°C, water bath warming, followed by intratesticular allotransplantation of neonatal rat testis results in the development of testicular interstitium and spermatogenic epithelium of donor organs into recipient body. Probl Cryobiol Cryomed 2020; 30(1): 34-46

Highlights

Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals
Cryopreservation of testicular tissue of endangered animal species and breeds having a tremendous agricultural value necessitates the establishment of germplasm banks [27, 33]
Histological examination of neonatal testis showed that it consisted of immature seminiferous tubules of 20–30 μm diameter (Fig. 1A)

Summary

Introduction

Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. V. Keros et al [15] has proven the efficiency of protective medium that contained DMSO, sucrose, bovine serum albumin and some other components, when using the programmable cooling, which comprises the crystallization initiation at –8°C, gradual cooling down to –80°C with varied cooling проблеми кріобіології і кріомедицини problems of cryobiology and cryomedicine том/volume 30, No/issue 1, 2020 по криоконсервированию фрагментов тестикул животных и человека. The experiments were performed in accordance with the Law of Ukraine 'On the Protection of Animals Against Cruelty' (No 3447–IV of February 21st, 2006), in compliance with the requirements проблеми кріобіології і кріомедицини problems of cryobiology and cryomedicine том/volume 30, No/issue 1, 2020 ных животных [34], на моделях ауто- или сингенной трансплантации фрагментов и клеток тестикул [28], что оставляет открытым вопрос об использовании аллотрансплантации.

Results

Conclusion