Abstract
An analysis of literature data the following preprocessing methods starch, pentosane- and cellulosic feedstock for enzymatic hydrolysis polysaccharides: hydrothermal, organosolvent obtaining technical cellulose, organosolvent with sulfuric acid, acid-catalyzed steam explosion, alkali, bisulfite (SPORL) and acid-catalyzed using a disk mill is presented.Recommendations about improvement of technological schemes of obtaining fodder protein products of bran together with other grain waste are provided. Technological scheme should include additional process fine grinding of bran and grain waste and hydro-thermodynamic treatment (HTDO) at the next technological mode: a.d.s ≥ 15%; t = 86–100 °C; processing time ≤ 1 h; using a thermostable α-amylase (Zymajunt-340С* or Amylase HT-4000 or Amileks 4T). The effectiveness of a subsequent enzymatic hydrolysis of partially decomposed starch and non-starch polysaccharides of starchy raw material is ensured by the enzymatic Начало формыКонец формыcomplex including Glucoamylase, GC 220, containing cellulase, cellobiase, xylanase and β-glucanase activities, and Novozymes 188, containing cellobiase.The most effective methods for preprocessing of pentosan - and cellulosic feedstock to enzymatic hydrolysis of cellulose in a 2% suspension of lignocellulosic residue are allow obtained an overall yield of sugars and ethanol at least 90% of the theoretical. The following preprocessing methods are considered to be as the most promising: organosolvent with H2SO4 and steam explosion with SO2 and the subsequent washing of the extruded biomass with water and oxidation-alkaline solution and mechanochemical: bisulfite (SPORL) and acid catalysis units with installation of hot grinding (IHG). It is established, that in the first two methods allowed the lignin content in the lignocellulose is to 16-18%, and the third up to 30%. Acid-catalyzed hydrolysis method with elements of SPORL technology with the use of installation hot grinding (IHG) is recommended for laboratory and experimental-industrial tests in the hydrolysis industry.Cellulose from lignocellulosic residue is efficiently hydrolysed by Сelluclast (20 FPU/g glucan), and Novozyme 188 (β-glucosidase : cellulase 1,75–2,0 : 1) or Spezyme CP, GC-220 (15 FPU/g glucan), Multifect Xylanase (β-glucosidase:cellulase 2,0 : 1).
Highlights
Candida retilis[13]. 24 ) [4, 8]. ( ). , : – 0,8) [4, 8]. [14–16]. [15]. (t = 180 200 °C (85%) [16]. 17% [17]. [2, 14, 17, 18]. 68–85% [2]. [2, 8, 9, 18–27]. [20, 21]. ». 1% [22]. [22–27]. [23]. (t = 135–180 ° ). t = 165–180 ° [24]. [25]. 1,9). [3, 23, 26]. ) [23]. [26]. [27]. ) [3, 24–27]. 10, 58,5%; 10,9) [29]. 6–7, 51,2%, 30, 3,1%) [28]; Organosell c (NaOH 17–22%, 25–40% ; t = 155-170 ° , = 60– 3–5, 22,1) [34, 35]. SEW. ASAM. [3, 36, 37]. [37]: Novozim 188), 2,0–3,4; t = 185–198 °C, 6,4, 18,4 9,2%
Sulfite cooking methanol for the production of cellulose from materials containing lignocellulose with recovery of the cooking chemicals / Rufolf Patt, Reinbek, Othar Kordsachia, Ostseinbek / 1988
Fast and efficient Alkaline Peroxide Treatment to enchance the Ensimatic digestibility of steam-exploded softwood substrates // Biotechnology and Bioengineering. 2002
Summary
[13]. 24 ) [4, 8]. ( ). , : – 0,8) [4, 8]. [14–16]. [15]. (t = 180 200 °C (85%) [16]. 17% [17]. [2, 14, 17, 18]. 68–85% [2]. [2, 8, 9, 18–27]. [20, 21]. ». 1% [22]. [22–27]. [23]. (t = 135–180 ° ). t = 165–180 ° [24]. [25]. 1,9). [3, 23, 26]. ) [23]. [26]. [27]. ) [3, 24–27]. 10, 58,5%; 10,9) [29]. 6–7, 51,2%, 30, 3,1%) [28]; Organosell c (NaOH 17–22%, 25–40% ; t = 155-170 ° , = 60– 3–5, 22,1) [34, 35]. SEW. ASAM. [3, 36, 37]. [37]: Novozim 188), 2,0–3,4; t = 185–198 °C, 6,4, 18,4 9,2%.
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