Abstract
Technological development of environmentally friendly biofuels is highly desirable due to the depletion of fossil fuel reserves and increased environmental awareness. Currently, bioethanol appears to be the key alternative fuel, most of which is made from sugar cane, corn, and wheat. However, the use of these sugar- and starch-basedmaterials is still controversial due to their alternative use as animal feed and as raw materials for human staple foods. Various detoxification strategies have been developed to remove these inhibitors from lignocellulose hydrolysates and reduce their levels, such as alkali treatment, sulfite treatment, evaporation, anion exchange and laccase addition.However, these methods create 2 main problems in terms of their technical and economic feasibility: the addition of expensive processing stages and the loss of easily digestible sugars.
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