Abstract
The article discusses the selection of optimal conditions for the lyophilization of poly- and monoclonal peroxidase conjugates used in the direct version of the enzyme immunoassay intended for the cholera toxin detection in Vibrio cholerae O1, O139 strains. Effective stabilizers included in protective media comprise 1 % sucrose, 1 % sodium thiosulfate, 0.5 % egg albumin, or 0.5 % bovine serum albumin. Their use increases the shelf life, increases stability, and contributes to the preservation of high sensitivity and specificity of poly- and monoclonal peroxidase conjugates. Evaluation of the serological activity of antitoxic conjugates in ELISA after lyophilization in a protective medium with stabilizers showed that it remains at the initial level. The results of testing lyophilized conjugates during storage allow us to speak about the possibility of their use for two years without changing the main indicators.
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