Abstract
Here, we have studied the impact of preliminary incubation of human and bovine erythrocytes in glycerol solutions on their sensitivity to hypertonic shock. The cell damage was shown to significantly reduce with increasing the glycerol concentration. The ranges of a sharp change in human and bovine cell sensitivity were from 0.1 to 0.5 and 0.5 to 1 M, respectively. A change in incubation time with glycerol from 1 to 10 min resulted in sensitivity decrease to hypertonic shock for both mammalian species, but the curves of dependence were different. A damaging effect of hypertonic shock increased with temperature rise from 0 up to 10°C for human erythrocytes and from 0 to 25°C for bovine ones. Preliminary erythrocyte incubation with glycerol entailed a decreased hypertonic damage of erythrocytes at all the studied temperatures for human cells and at those above 5°C for bovine cells. Our findings and the reported data review suggested about a different protective mechanism for human and bovine erythrocytes using glycerol under hypertonic shock conditions. Probl Cryobiol Cryomed 2019; 29(3):199–205.
Highlights
In this study we have studied the impact of preliminary incubation of human and bovine erythrocytes in glycerol solutions on their sensitivity to hypertonic shock
We have studied a hypertonic shock to simulate the impact of hypertonic saline on cells as one of the main factors of cryodamage [1]
The penetrating cryoprotectant glycerol was established to have a protective effect under hypertonic shock as for human and bovine erythrocytes
Summary
In this study we have studied the impact of preliminary incubation of human and bovine erythrocytes in glycerol solutions on their sensitivity to hypertonic shock. The ranges of cryoprotectant concentrations, when a sharp change in cell sensitivity occurred, were from 0.1 to 0.5 and 0.5 to 1 M for human and bovine erythrocytes, respectively. The incubation of both mammalian species erythrocytes with glycerol for 10 min reduced their sensitivity to hypertonic shock. For human erythrocytes, this index decreased gradually, and for bovine ones the dependence was not monotone.
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