ПАРАМЕТРЫ ЭРИТРОПОЭЗА И ЦИТОАРХИТЕКТОНИКА ЦИРКУЛИРУЮЩИХ ЭРИТРОЦИТОВ КРЫС С АСЦИТНОЙ ОПУХОЛЬЮ ЯИЧНИКОВ

Abstract

Developing neoplasm can affect the erythropoiesis of the tumor-bearer. It is found out that the degree and nature of such effect depend on tumor biological characteristics. The purpose of the study is to examine erythrocyte morphometric parameters and erythropoiesis change in tumor-bearing animals under neoplastic process development. Materials and Methods. Peripheral blood of experimental animals was used as trial material. Blood sampling was carried out during stationary (n=64) and terminal (n=64) tumor growth phases. Healthy adult rats (n=62) composed the control group. To assess erythropoiesis, the authors determined erythrocyte content, hemoglobin level, reticulocyte count, and erythropoietin concentration in the peripheral blood of tumor-bearing animals. Cytoarchitectonics was studied using atomic force microscopy. Results. Significant reduction in the number of red blood cells and decrease in hemoglobin level was established. The erythropoietin level in blood plasma increased during the stationary phase as well as the number of reticulocytes in blood during all periods of tumor growth. Erythrocyte cytoarchitectonics changed: echinocytes prevailed in the stationary phase (52%), spherocytes – in the terminal phase (50.8 %). Conclusion. The data obtained suggest that, on the background of intense erythropoiesis, the number of erythrocytes in peripheral blood falls, the hemoglobin level decreases, there is some change in cytoarchitectonics, which may indicate a toxic effect of the tumor on the hematopoietic system. Keywords: erythrocytes, erythropoietin, ovarian ascites tumor.