ОЦЕНКА ЭФФЕКТИВНОСТИ УНИВЕРСАЛЬНОЙ ВАКЦИНЫ ПРОТИВ ХЛАМИДИО-ЗА СЕЛЬСКОХОЗЯЙСТВЕННЫХ ЖИВОТНЫХ НА КРОЛИКАХ

Abstract

The article presents data on laboratory tests of a universal vaccine against chlamydia of farm animals for sterility, safety, antigenic activity and immunogenicity. The sterility of the new vaccine preparation was evaluated on nutrient media (MPA, MPB, MPPB and Saburo), the vaccine was harmless according to the results of bioassays on laboratory white mice and guinea pigs. The vaccine was found to be sterile and harmless to laboratory animals. Evaluation of antigenic activity was car-ried out on rabbits, the study lasted 195 days, the results were taken into account on the basis of data obtained during systematic serological studies of blood serum immunized with rabbits. It was found that the vaccine stimulates the immune system of laboratory animals to produce complemen-tary chlamydial antibodies. After 6.5 months after the administration of the drug, the titers of chla-mydial antibodies remained above the protective titer and were equal on average 1:60. Evaluation of the immunogenicity of the vaccine was carried out in an acute experiment on rabbits (females). Dur-ing the experiment, it was possible to establish the ability of the vaccine to induce the production of immunity in laboratory animals that can overcome chlamydial infection. Healthy rabbits were ob-tained from rabbits vaccinated and infected at the 3rd week of pregnancy, while mass abortions, stillbirths and the birth of non-viable offspring were noted in the control groups. According to the results of post-mortem dissection of animals from the experimental and control groups, it was found that vaccinated and further infected animals did not reveal any pathological changes in the internal organs, in contrast to the animals from the control groups.