Циркулирующие опухолевые клетки: мониторинг течения рака молочной железы в реальном времени

Abstract

The aim of the research. Th is study aims to determine the content of circulating tumor cells (CTCs) in the peripheral blood using the MDA 231 aptamer in the dynamics of the disease. Material and methods. Th e study included 6 patients with breast cancer, who have been under dispensary supervision since 2017. Detection of CTCs was carried out in 3.5 ml of the blood of BC patients with the help of the MDA 231 aptamer which is affi ne for breast cancer cells, labeled with the fl uorescent Cy-5 dye. Th e count of CTCs in the blood samples was performed using fl uorescent and laser scanning microscopy. Results. Using the MDA231 aptamer, the content of CTCs in the peripheral blood of patients with breast cancer in the dynamics of the disease was analyzed. CTCs were detected in 1 patient, who was subsequently diagnosed with metastatic liver damage. Conclusion. Th e study demonstrates the possibility of using aptamers to determine the risk of relapse and to evaluate the eff ectiveness of antitumor therapy in patients with breast cancer.