섬유소에서 추출한 베타-포도당의 세포 내 에너지원으로의 대체 가능성에 관한 연구

Abstract

The present study investigated the application possibility of β-glucose derived from plant cellulose for a cellular energy source. The β-glucose was firstly extracted by the cellulase treatment from filter paper. The A549, AGS and HCT-116 cancer cell lines was properly proliferated in the DMEM containing 2% serum and 4.5 g/L glucose. Following cancer (A549, AGS and HCT-116), and normal (MRC-5 and DSC) cells treatment in the β-glucose DMEM media, the rate of glucose uptake and cell growth was similar to that of the α-glucose DMEM media, implying that the β-glucose could be used for cellular energy source. Moreover, the differentiation capacity of cancer cells (A549, AGS and HCT-116) into adipocytes in the β-glucose DMEM media was also similar to that of the α-glucose DMEM media, implying that the β-glucose could be also used for energy storage source. Taken together, we concluded that β-glucose derived from plant cellulose could be used as a potential source of cellular energy and storage, like starch α-glucose from starch.