Показники прооксидантно-антиоксидантної системи печінки при експериментальному антифосфоліпідному синдромі та застосуванні L-аргініну

Abstract

Introduction. Antiphospholipid syndrome (APS) that is developed due to underlying autoimmune mechanism often causes miscarriage, vessels thrombosis of different localization, cerebrovascular accident, cardiovascular and other diseases that are accompanied by significant activation of free radical processes. Pathogenesis of liver damage in cases of APS has not been adequately studied.The aim of the study – to establish the changes in parameters of prooxidative-antioxidant system and tissue respiration in liver in cases of experimental antiphospholipid syndrome and L-arginine administration.Research Methods. Mice of BALB/c line were used in the research. The APS was modeled using cardiolipin emulsified in 75 complete Freund's adjuvant; subsequent injections were administered with incomplete Freund's adjuvant. Cardiolipin was injected intramuscularly four times at a dose of 30 µg per 1 injection with 14-day intervals between the injections. For correction, L-arginine hydrochloride (25 mg/kg) was administered once daily during 10 days after APS development.The activity and content of antioxidant system components (superoxide dismutase, catalase, reduced glutathione) were investigated in liver tissue. The level of lipid peroxidation products was determined by the content of lipid hydroperoxides and TBA-active products. The state of energy-supplying processes of mitochondria was studied due to the activity of succinate dehydrogenase and cytochrome oxidase. The concentration of proteins was determined by Lowry method.Results and Discussion. In cases of APS in liver the imbalance in functioning of prooxidative antioxidant system (increased activity of superoxide dismutase and catalase, decrease in the content of reduced glutathione, increase in the level of lipids hydroperoxides, TBA-active products), of mitochondrial electron transport chain (decrease in the activity of succinate dehydrogenase, cytochrome oxidase) in cases of experimental antiphospholipid syndrome was evidenced. In cases of experimental APS in presence of L-arginine administration, the re-establishment of activity and content of antioxidant system components, enzymes of mitochondrial electron transport chain, decrease in content of lipid hydroperoxides and TBA-active products in liver was proved.Conclusion. In cases of experimental antiphospholipid syndrome, L-arginine, in the event of its repeated administration, reduces manifestations of oxidative stress and reactivate enzymes of mitochondrial electron transport chain in liver tissue of BALB/c mice.