나도 풍란을 이용한 속간 교잡종의 수정 및 배형성과 종자 발달에 대한 연구

Abstract

On 5-7 days after artificial pollination using NadoPungRan(Phalenopsis japonica), native orchid in Korea, pollen were germinated in sticky stigma and pollen tube elongation in style were observed. on 14 days after pollination. In this period, ovule primodia appeared to round pronunciation shape. But filament shape and Megaspore Mother Cell were appeared on 34 days after pollination. Ovule which is ready fertilization, was matured in 40-50 days after pollination. In these time, pollen tube elongation were continued and entirely surrounded ovule sac. On 54-64 days after pollination, pollen tube penetrated to ovule sac and fertilization were completed. After fertilization, zygote was divided to two cell in longitudinal division and upper small cell was developed into proembryo and bottom large cell divided two cell which make suspensor cell. On 110 days after pollination, matured embryo and suspensor which have 16 filament leg, were observed and seed were matured on about 140 days after pollination. To breed intergeneric hybrids between NadoPungRan and Moth orchid, pods were collected in week after pollination and seed germination using artificial medium were tried. Young ovules before 56 days after pollination were not germinated. Ovules on 69 days after pollination were good for germination and to get hybrids.