Abstract
The research was performed to reveal an antihemolytic effect of chlorpromazine (CPZ) under post-hypertonic stress (PHS) of human erythrocyte depending on the substances present at different stages of experiment (pre-treatment, dehydration, rehydration) as well as the effect of CPZ on the redistribution of phosphatidylserine in erythrocyte membrane bilayer. It has been shown that pre-treatment of erythrocytes with CPZ at a concentration of 180 mmol/l did not lead to antihemolytic effect of the substance under PHS of erythrocytes. It has been established that CPZ under concentration of 100-300 mmol/l did not cause a transbilayer redistribution of phosphatidylserine molecules in erythrocyte membranes. Protective effect of CPZ was implemented following transfer of the cells from the dehydration medium (1.75 mol/l NaCl) into rehydration one (0.15 mol/l NaCl), containing CPZ, i. e.at the moment of stress action. Consequently, the mechanism of antihemolytic action of CPZ under PHS of erythrocytes was associated with the membrane reorganization during an incorporation of the substance molecules into it. Probl Cryobiol Cryomed 2017; 27(3): 219–229
Highlights
The research was performed to reveal an antihemolytic effect of chlorpromazine (CPZ) under posthypertonic stress (PHS) of human erythrocyte depending on the substance presence at different stages of experiment as well as the effect of CPZ on the redistribution of phosphatidylserine in erythrocyte membrane bilayer
Erythrocytes exposed to the effect of stress factors can react to the altered morphological characteristics of cells maintaining the integrity of plasma membrane
In a recently published report, we have shown that the use of CPZ during PHS of human erythrocytes allowed to reduce the level of hemolytic damage [29]
Summary
The research was performed to reveal an antihemolytic effect of chlorpromazine (CPZ) under posthypertonic stress (PHS) of human erythrocyte depending on the substance presence at different stages of experiment (pre-treatment, dehydration, rehydration) as well as the effect of CPZ on the redistribution of phosphatidylserine in erythrocyte membrane bilayer. It has been shown that pre-treatment of erythrocytes with CPZ at a concentration of 180 μmol/L did not lead to antihemolytic effect of the substance under PHS of erythrocytes. The mechanism of antihemolytic action of CPZ under PHS of erythrocytes was associated with the membrane reorganization during an incorporation of the substance molecules into it. If a specific threshold value of the stress factor is exceeded, the membrane barrier function will become compromized, and the cells will.
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