Физико-химический анализ гидрофильных интраокулярных линз, эксплантированных в связи с помутнением материала

Abstract

Relevance. Clouding and discoloration of intraocular lens (IOL) seems to be a multifactorial problem, directly related to the properties of each group of materials from which they are made. Since the exact combination of factors and the sequence of events are not yet fully known, there is reason to continue research in this direction, even though, unfortunately, such important points as the specifics of IOL production are information closed to the general public. It is also obvious that this problem is urgent and requires an integrated approach. Purpose. To determine the type of deposits on the surface of hydrophilic IOLs explanted due to a decrease in the transparency of the material. Material and methods. Visualization of the surface of 8 explanted hydrophilic IOLs was carried out in a digital inverted microscope Altami INVERT 3 (LLC Altami, Russia). Elemental analysis of surface deposits was carried out on a JCM-6000 electron microscope (Jeol, Japan) with a JED-2300 energy dispersive analysis attachment. The Raman spectra were measured using M532 Raman microscope (Enspectr, USA). Infrared absorption spectra (IR spectra) were recorded using equipment: Fourier spectrometer InfraLum FT-02 (Lumex, Russia) and an IR spectrometer with an attachment of frustrated total internal reflection IRTracer-100 (Shimadzu, Japan). The processing and interpretation of the obtained spectra was carried out using databases and software from the equipment. Results. The nature of the deposits is represented by various crystallites, in some places forming a completely continuous film. The main elements of deposits are carbon, nitrogen, and oxygen. The IR spectrum contains functional groups characteristic of proteins. Conclusion. Deposits on the surface of a cloudy IOL of a hydrophilic type are of protein nature. The fact that the deposits have a crystalline structure makes it possible to significantly narrow the scope of the search for those compounds that are capable of crystallizing at temperatures close to the normal temperature of the human body. Key words: IOL clouding, hydrophilic IOL, IOL explantation