Экспериментальное обоснование антисурдитантного применения производного триазино-индола при воздействии шума высокой интенсивности

Abstract

In the study on the model of acoustic trauma in experimental animals (mice), the otoprotective effect of the triazine-indole derivative was shown due to the influence on the expression level of hypoxia-inducible factor (HIF) in the Corti organ and spiral ganglion cells. For therapeutic purposes, the drug was administered as a 1% solution at a dosage of 5, 7, 10 mg/kg 2 hours after acoustic exposure. For preventive purposes, the drug was administered at a dosage of 5, 7, 10 mg/kg for 3 days before acoustic exposure. The comparator drug was Cytoflavin. The control group received a saline solution. The immunohistochemical study was carried out using an anti-mouse polymerized immunohistochemical imaging system and mouse monoclonal antibodies to HIF1. In immunohistochemical preparations scanned using a digital scanner, the HIF1-positive area (%) in the cochlea and spiral ganglion cells was calculated using a morphometric program. The color was evaluated by the average cytochemical coefficient. It was found that the severity of HIF expression depended on the dose of the administered drug and coincided with electrophysiological changes in experimental animals. The antisurditant effect of the triazine-indole derivative is realized by influencing the level of HIF expression, which allows it to be regarded as a target molecule when using the drug.