Влияние гормона роста человека на состояние коллагена в области повреждения кожи крыс

Abstract

Investigation of opportunities to affect the synthesis of collagen is of current importance to surgery as the synthesis of extracellular matrix collagen is the constituent of wound healing, which determines the rate and quality of tissue restoration. Growth hormone is one of the factors influencing the synthesis of the major protein of connective tissue - collagen. We have hypothesized that after making a subcutaneous wound, by impairing the basement membrane integrity, and by causing skin defect resolution, a recombinant human growth hormone (rh GH) preparation may affect the starting synthesis of an extracellular collagen matrix. An electric dermatome was used for partial skin thickness damage. The experimental animals - Wistar rats were randomized into two groups. The experimental group received subcutaneous rhGH injections (2.0 mg/kg) every day. The control group was daily given subcutaneous placebo injections. Wound tissue histomorphometry was used to estimate the area of the dermal fibrillary collagen network, the number of fascicles and the length of their perimeter and to calculate the volume of the fibrillary collagen network. There were both a statistically significant prevalence of collagen synthesis in the matrix of a wound on days 3, 6, 9 (66.55tl2.65, 64.23±7.19, and 65.74±8.I8) versus (51.32+- 14.85; 52.77+-2.41 , and 61.53±9.35), respectively, and signs of early mature connective tissue formation in the region of partial skin thickness damage in the rats receiving rh GH injections, which manifested as fewer collagen fibrils (156.23±5.53 and 166.14±4.24 to 143.34±2.90) as compared with the controlgroup (200.13±4.6S and 193.79+-.5.35 to I54.14±5.42, respectively).